How to prove Orlistat is an inhibitor for lipase?
I need to design an experiment to prove that Orlistat is an inhibitor of lipase. Do you have any suggestion on how i might start?
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- Well, if it's an in vittro experiment , get some lipase (sold as pancreatic enzyme) and place lipase in 2 test tubes. Add orlistat to one and add lipids to both shake and see the results. If it is an in vivo experiment, get several of your friends to eat a high fat meal. Give half orlistat. See the results....you'd expect smelly,fatty stools that float in the group that took orlistat and normal stools in those that didn't.
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